The Flow Cytometry Facility provides resources for analytical and preparative studies of cells using flow cytometry to the FAMU biomedical researchers. This facility offers critically important technology for gaining information about normal versus disease states. The utilization of in vivo (animals and human) or in vitro studies to characterize structures or visualize molecular markers using various image methods is a powerful tool in clinical and biomedical research. The facility will provide resources for image data acquisition and analysis for radioactive, fluorescent, and photographic samples as well as digital imaging and document production. The facility also offers FACS acquisition and analysis for detecting cell surface or intracellular proteins, measuring apoptosis, cell cycle analysis and progression, and cellular physiology that have both biomedical and clinical applications.
Specific Services: Services include: a) Operator assisted acquisition and analysis; b) Operator unassisted analysis and acquisition (for individuals who have attended an instructional class); c) Off-line data analysis; d) Cell cycle analysis; e) Calcium flux analysis; f) Receptor Endocytosis; g) Sample staining and analysis; h) Cytometric Bead Arrays; i) Annexin V for apoptosis; j) Multiplexed analysis of signal transduction; k) Phosphorylation target assays; l) Detection of Cell Surface Markers; m) Detection of Intracellular Markers; n) Sorting cell populations of interest o) preparing specimens (i.e. Hematoxylin & Eosin Staining, Single Cell Gel Electrophoresis staining (Comet Assay), fluorescent conjugates, tissue fixing, and immunohistochemistry); p) image processing; and q) data acquisition and analysis for radioactive, fluorescent, and photographic sample.
Badisa, Ramesh, Ph.D.
Role: Research Associate
Access to flow cytometer.