This is a summary list of all core laboratories at Florida Agricultural and Mechanical University . The list includes links to more detailed information, which may also be found using the eagle-i search app.
Molecular Modeling Facility
The Molecular Modeling Facility provides the modern modeling software suites on Windows and Linux platforms and technical assistance in performing QSAR, Protein receptor ligand docking, Virtual screening, Cheminformatics, Molecular Dynamics and
other functions as required for drug discovery This facility will be instrumental in assisting with the research conducted in the newly proposed FAMU-RCMI Cancer and Neuroscience Cores
The NMR Facility provides instrumentation and technical assistance for obtaining NMR (Nuclear Magnetic Resonance) spectra for liquid samples. This facility supports the drug discovery research and other chemical, biochemical researches to monitor chemical reactions, identify / elucidate chemical structures, and to determine purity of synthetic products. This facility also provides basic training for graduate students and undergraduate students both in the college of pharmacy and in the chemistry department on how to use the instrument to meet their research / experimental needs, as well as the requirements for medicinal chemistry or organic chemistry courses.
The Flow Cytometry Facility provides resources for analytical and preparative studies of cells using flow cytometry to the FAMU biomedical researchers. This facility offers critically important technology for gaining information about normal versus disease states. The utilization of in vivo (animals and human) or in vitro studies to characterize structures or visualize molecular markers using various image methods is a powerful tool in clinical and biomedical research. The facility will provide resources for image data acquisition and analysis for radioactive, fluorescent, and photographic samples as well as digital imaging and document production. The facility also offers FACS acquisition and analysis for detecting cell surface or intracellular proteins, measuring apoptosis, cell cycle analysis and progression, and cellular physiology that have both biomedical and clinical applications.
Specific Services: Services include: a) Operator assisted acquisition and analysis; b) Operator unassisted analysis and acquisition (for individuals who have attended an instructional class); c) Off-line data analysis; d) Cell cycle analysis; e) Calcium flux analysis; f) Receptor Endocytosis; g) Sample staining and analysis; h) Cytometric Bead Arrays; i) Annexin V for apoptosis; j) Multiplexed analysis of signal transduction; k) Phosphorylation target assays; l) Detection of Cell Surface Markers; m) Detection of Intracellular Markers; n) Sorting cell populations of interest o) preparing specimens (i.e. Hematoxylin & Eosin Staining, Single Cell Gel Electrophoresis staining (Comet Assay), fluorescent conjugates, tissue fixing, and immunohistochemistry); p) image processing; and q) data acquisition and analysis for radioactive, fluorescent, and photographic sample.
The Proteomics Facility provides a variety of proteomic services including protein molecular weight determination, identification and analysis. This facility will enable FAMU Biomedical Researchers to answer pressing biological questions about the functions and roles of proteins in diseases impacting the underserved and minority populations. With sample preparation, separations, mass spectrometry, and informatics, we will be able to provide information that drives knowledge about disease pathology and target based drug discovery will be realized.
• Protein separation using either 2D-Gel electrophoresis
• Gel imaging using Bio-Rad VersaDoc Imager
• Archiving and retrieval of proteomics data for protein informatics analysis
• Sample preparation
• Provide consultation on experimental design
• Provide individual/group proteomics training
• Digest samples analyzed using ABSciex 4800 MALDI-TOF-TOF
• Gel spot cutting, trypsin digestion and peptide extraction
Found 5 core laboratories .